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tandem fluorescent reporter plasmid pcdna3 gfp lc3 rfp lc3δg  (Addgene inc)


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    Addgene inc tandem fluorescent reporter plasmid pcdna3 gfp lc3 rfp lc3δg
    Tandem Fluorescent Reporter Plasmid Pcdna3 Gfp Lc3 Rfp Lc3δg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+wnt8/ZP43+Wnt8+3'+orf1+(Plasmid+%2316899)/pm40818359-60-17-22
    Average 93 stars, based on 14 article reviews
    tandem fluorescent reporter plasmid pcdna3 gfp lc3 rfp lc3δg - by Bioz Stars, 2026-09
    93/100 stars

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    Expressing:

    Article Title: ERAD‐dependent control of the Wnt secretory factor Evi
    Article Snippet: .. The following expression constructs were used: pSpCas9(BB)‐2A‐Puro (px459; Addgene #48139; Ran et al , ), psPAX2 (Addgene #12260; D. Trono), pMD2.G‐VSVG (Addgene #12259; D. Trono), TCF4/Wnt‐Firefly Luciferase (Demir et al , ), Actin‐Renilla Luciferase (Nickles et al , ), pcDNA3‐Wnt11 (Addgene #35922; Najdi et al , ), pcDNA3‐Wnt10B, (Addgene #35921; Najdi et al , ), pcDNA3‐Wnt16 (Addgene #35923; Najdi et al , ), pcDNA3‐Wnt7B (Addgene #35915; Najdi et al , ), pcDNA3‐Wnt8 (Addgene #35916; Najdi et al , ), pcDNA3‐Wnt5A (Addgene #35911; Najdi et al , ), pcDNA3‐Wnt3A (Addgene #35908; Najdi et al , ), pcDNA3‐Wnt3 (Addgene #35909; Najdi et al , ), pcDNA3.2‐Wnt3A‐V5 (Addgene #43810; MacDonald et al , ), pcDNA3‐IGFBP5‐V5 (Addgene #11608; S. Johnson), pcDNA3‐EGF‐Myc (Addgene #11601; Nguyen et al , ), VCP(wt)‐EGFP (Addgene #23971; Tresse et al , ), VCP(DKO)‐EGFP (Addgene #23974; Tresse et al , ), pCMV6‐Myc‐DDK‐tagged PORCN (Origene # RC223764), sLuc (Katanaev et al , ), pCS2(+)‐mWnt3A (C. Niehrs), pCS2(+)‐Dvl3 (C. Niehrs), pCS2(+)‐mWnt3A‐S209A (Kumar et al , ), and β‐Catenin‐YFP (Stemmer et al , ). .. To generate C‐terminally FLAG‐HA‐tagged CGRRF1 and Hrd1, the FLAG‐HA sequence was inserted into the pcDNA5‐FRT/TO vector (Invitrogen).

    Article Title: ERAD-dependent control of the Wnt secretory factor Evi.
    Article Snippet: .. The following expression constructs were used: pSpCas9(BB)-2A-Puro (px459; Addgene #48139; Ran et al, 2013), psPAX2 (Addgene #12260; D. Trono), pMD2.G-VSVG (Addgene #12259; D. Trono), TCF4/Wnt-Firefly Luciferase (Demir et al, 2013), Actin-Renilla Luciferase (Nickles et al, 2012), pcDNA3-Wnt11 (Addgene #35922; Najdi et al, 2012), pcDNA3-Wnt10B, (Addgene #35921; Najdi et al, 2012), pcDNA3Wnt16 (Addgene #35923; Najdi et al, 2012), pcDNA3-Wnt7B (Addgene #35915; Najdi et al, 2012), pcDNA3-Wnt8 (Addgene 14 of 19 The EMBO Journal 37: e97311 | 2018 a 2018 The Authors D ow nloaded from https://w w w .em bopress.org on M arch 25, 2024 from IP 2401:4900:1ce0:5304:ec18:f025:b163:a1a2. .. #35916; Najdi et al, 2012), pcDNA3-Wnt5A (Addgene #35911; Najdi et al, 2012), pcDNA3-Wnt3A (Addgene #35908; Najdi et al, 2012), pcDNA3-Wnt3 (Addgene #35909; Najdi et al, 2012), pcDNA3.2-Wnt3A-V5 (Addgene #43810; MacDonald et al, 2014), pcDNA3-IGFBP5-V5 (Addgene #11608; S. Johnson), pcDNA3-EGFMyc (Addgene #11601; Nguyen et al, 2000), VCP(wt)-EGFP (Addgene #23971; Tresse et al, 2010), VCP(DKO)-EGFP (Addgene #23974; Tresse et al, 2010), pCMV6-Myc-DDK-tagged PORCN (Origene # RC223764), sLuc (Katanaev et al, 2008), pCS2(+)-mWnt3A (C. Niehrs), pCS2(+)-Dvl3 (C. Niehrs), pCS2(+)-mWnt3A-S209A (Kumar et al, 2014), and b-Catenin-YFP (Stemmer et al, 2008).

    Construct:

    Article Title: ERAD‐dependent control of the Wnt secretory factor Evi
    Article Snippet: .. The following expression constructs were used: pSpCas9(BB)‐2A‐Puro (px459; Addgene #48139; Ran et al , ), psPAX2 (Addgene #12260; D. Trono), pMD2.G‐VSVG (Addgene #12259; D. Trono), TCF4/Wnt‐Firefly Luciferase (Demir et al , ), Actin‐Renilla Luciferase (Nickles et al , ), pcDNA3‐Wnt11 (Addgene #35922; Najdi et al , ), pcDNA3‐Wnt10B, (Addgene #35921; Najdi et al , ), pcDNA3‐Wnt16 (Addgene #35923; Najdi et al , ), pcDNA3‐Wnt7B (Addgene #35915; Najdi et al , ), pcDNA3‐Wnt8 (Addgene #35916; Najdi et al , ), pcDNA3‐Wnt5A (Addgene #35911; Najdi et al , ), pcDNA3‐Wnt3A (Addgene #35908; Najdi et al , ), pcDNA3‐Wnt3 (Addgene #35909; Najdi et al , ), pcDNA3.2‐Wnt3A‐V5 (Addgene #43810; MacDonald et al , ), pcDNA3‐IGFBP5‐V5 (Addgene #11608; S. Johnson), pcDNA3‐EGF‐Myc (Addgene #11601; Nguyen et al , ), VCP(wt)‐EGFP (Addgene #23971; Tresse et al , ), VCP(DKO)‐EGFP (Addgene #23974; Tresse et al , ), pCMV6‐Myc‐DDK‐tagged PORCN (Origene # RC223764), sLuc (Katanaev et al , ), pCS2(+)‐mWnt3A (C. Niehrs), pCS2(+)‐Dvl3 (C. Niehrs), pCS2(+)‐mWnt3A‐S209A (Kumar et al , ), and β‐Catenin‐YFP (Stemmer et al , ). .. To generate C‐terminally FLAG‐HA‐tagged CGRRF1 and Hrd1, the FLAG‐HA sequence was inserted into the pcDNA5‐FRT/TO vector (Invitrogen).

    Article Title: ERAD-dependent control of the Wnt secretory factor Evi.
    Article Snippet: .. The following expression constructs were used: pSpCas9(BB)-2A-Puro (px459; Addgene #48139; Ran et al, 2013), psPAX2 (Addgene #12260; D. Trono), pMD2.G-VSVG (Addgene #12259; D. Trono), TCF4/Wnt-Firefly Luciferase (Demir et al, 2013), Actin-Renilla Luciferase (Nickles et al, 2012), pcDNA3-Wnt11 (Addgene #35922; Najdi et al, 2012), pcDNA3-Wnt10B, (Addgene #35921; Najdi et al, 2012), pcDNA3Wnt16 (Addgene #35923; Najdi et al, 2012), pcDNA3-Wnt7B (Addgene #35915; Najdi et al, 2012), pcDNA3-Wnt8 (Addgene 14 of 19 The EMBO Journal 37: e97311 | 2018 a 2018 The Authors D ow nloaded from https://w w w .em bopress.org on M arch 25, 2024 from IP 2401:4900:1ce0:5304:ec18:f025:b163:a1a2. .. #35916; Najdi et al, 2012), pcDNA3-Wnt5A (Addgene #35911; Najdi et al, 2012), pcDNA3-Wnt3A (Addgene #35908; Najdi et al, 2012), pcDNA3-Wnt3 (Addgene #35909; Najdi et al, 2012), pcDNA3.2-Wnt3A-V5 (Addgene #43810; MacDonald et al, 2014), pcDNA3-IGFBP5-V5 (Addgene #11608; S. Johnson), pcDNA3-EGFMyc (Addgene #11601; Nguyen et al, 2000), VCP(wt)-EGFP (Addgene #23971; Tresse et al, 2010), VCP(DKO)-EGFP (Addgene #23974; Tresse et al, 2010), pCMV6-Myc-DDK-tagged PORCN (Origene # RC223764), sLuc (Katanaev et al, 2008), pCS2(+)-mWnt3A (C. Niehrs), pCS2(+)-Dvl3 (C. Niehrs), pCS2(+)-mWnt3A-S209A (Kumar et al, 2014), and b-Catenin-YFP (Stemmer et al, 2008).

    Luciferase:

    Article Title: ERAD‐dependent control of the Wnt secretory factor Evi
    Article Snippet: .. The following expression constructs were used: pSpCas9(BB)‐2A‐Puro (px459; Addgene #48139; Ran et al , ), psPAX2 (Addgene #12260; D. Trono), pMD2.G‐VSVG (Addgene #12259; D. Trono), TCF4/Wnt‐Firefly Luciferase (Demir et al , ), Actin‐Renilla Luciferase (Nickles et al , ), pcDNA3‐Wnt11 (Addgene #35922; Najdi et al , ), pcDNA3‐Wnt10B, (Addgene #35921; Najdi et al , ), pcDNA3‐Wnt16 (Addgene #35923; Najdi et al , ), pcDNA3‐Wnt7B (Addgene #35915; Najdi et al , ), pcDNA3‐Wnt8 (Addgene #35916; Najdi et al , ), pcDNA3‐Wnt5A (Addgene #35911; Najdi et al , ), pcDNA3‐Wnt3A (Addgene #35908; Najdi et al , ), pcDNA3‐Wnt3 (Addgene #35909; Najdi et al , ), pcDNA3.2‐Wnt3A‐V5 (Addgene #43810; MacDonald et al , ), pcDNA3‐IGFBP5‐V5 (Addgene #11608; S. Johnson), pcDNA3‐EGF‐Myc (Addgene #11601; Nguyen et al , ), VCP(wt)‐EGFP (Addgene #23971; Tresse et al , ), VCP(DKO)‐EGFP (Addgene #23974; Tresse et al , ), pCMV6‐Myc‐DDK‐tagged PORCN (Origene # RC223764), sLuc (Katanaev et al , ), pCS2(+)‐mWnt3A (C. Niehrs), pCS2(+)‐Dvl3 (C. Niehrs), pCS2(+)‐mWnt3A‐S209A (Kumar et al , ), and β‐Catenin‐YFP (Stemmer et al , ). .. To generate C‐terminally FLAG‐HA‐tagged CGRRF1 and Hrd1, the FLAG‐HA sequence was inserted into the pcDNA5‐FRT/TO vector (Invitrogen).

    Article Title: ERAD-dependent control of the Wnt secretory factor Evi.
    Article Snippet: .. The following expression constructs were used: pSpCas9(BB)-2A-Puro (px459; Addgene #48139; Ran et al, 2013), psPAX2 (Addgene #12260; D. Trono), pMD2.G-VSVG (Addgene #12259; D. Trono), TCF4/Wnt-Firefly Luciferase (Demir et al, 2013), Actin-Renilla Luciferase (Nickles et al, 2012), pcDNA3-Wnt11 (Addgene #35922; Najdi et al, 2012), pcDNA3-Wnt10B, (Addgene #35921; Najdi et al, 2012), pcDNA3Wnt16 (Addgene #35923; Najdi et al, 2012), pcDNA3-Wnt7B (Addgene #35915; Najdi et al, 2012), pcDNA3-Wnt8 (Addgene 14 of 19 The EMBO Journal 37: e97311 | 2018 a 2018 The Authors D ow nloaded from https://w w w .em bopress.org on M arch 25, 2024 from IP 2401:4900:1ce0:5304:ec18:f025:b163:a1a2. .. #35916; Najdi et al, 2012), pcDNA3-Wnt5A (Addgene #35911; Najdi et al, 2012), pcDNA3-Wnt3A (Addgene #35908; Najdi et al, 2012), pcDNA3-Wnt3 (Addgene #35909; Najdi et al, 2012), pcDNA3.2-Wnt3A-V5 (Addgene #43810; MacDonald et al, 2014), pcDNA3-IGFBP5-V5 (Addgene #11608; S. Johnson), pcDNA3-EGFMyc (Addgene #11601; Nguyen et al, 2000), VCP(wt)-EGFP (Addgene #23971; Tresse et al, 2010), VCP(DKO)-EGFP (Addgene #23974; Tresse et al, 2010), pCMV6-Myc-DDK-tagged PORCN (Origene # RC223764), sLuc (Katanaev et al, 2008), pCS2(+)-mWnt3A (C. Niehrs), pCS2(+)-Dvl3 (C. Niehrs), pCS2(+)-mWnt3A-S209A (Kumar et al, 2014), and b-Catenin-YFP (Stemmer et al, 2008).



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